World’s 3rd Cloned Buffalo ‘GARIMA-II’ born at Karnal


World’s 3rd Cloned Buffalo ‘GARIMA-II’ born at Karnal


GARIMA-II, another cloned buffalo calf, through the new and advanced ‘Hand-guided Cloning Technique’ was born at Karnal based National Dairy Research Institute under  Indian Council of Agricultural Research on Sunday (August 22, 2010). This cloned buffalo calf is different from the earlier clone calf because, in this case, the used donor cell was embryonic stem cell, informed  Dr. A. K. Srivastava, Director, National Dairy Research Institute (NDRI) at Karnal.
World’s 3rd Cloned Buffalo ‘GARIMA-II’ born at KarnalThe world's first buffalo calf through the “Handguided Cloning Technique” was born on 6 February, 2009 at NDRI, Karnal and subsequently, the second cloned calf “GARIMA” was born on 6 June, 2009 with the birth weight of 43 kg. In earlier cloning, the donor cell was from somatic cells. The donor embryonic stem cell was isolated from the 8th day old blastocyst. These cells were cultured up to 29-passages (117 days) till it expressed pluripotent marker and then confirmed to be stem cell.
Dr. S. Ayyappan, Secretary DARE and Director General, Indian Council of Agricultural Research congratulated the team and said that the new technology of “Hand-guided Cloning” of buffaloes may lead a new era in faster multiplication of elite germplasm to face the challenges of increasing demands of milk in view of the ever growing human population.  Dr. K. M. L Pathak, Deputy Director General (Animal Sciences) and Dr. C. S. Prasad, ADG(AS) ICAR also congratulated the team.
Dr. A. K. Srivastava, Director emphasized that this technology could go a long way in helping for faster multiplication of superior milch buffaloes in India.  He said that although the world’s largest population of buffaloes is in India and they are contributing about 55% of total milk production in country, but the percentage of elite animals is very low and there is an urgent need to enhance the population of these elite buffaloes. He further emphasized that there is an acute shortage of good bulls and the technology of cloning will decrease this gap between  supply  and demand of breeding the bulls in the shortest possible time.
The team of the jubilant scientists involved in the production of this cloned calf using embryonic stem-cell as donor cell are Dr. M. S. Chauhan,  Dr. S. K. Singla,  Dr. R. S. Manik, Dr. P. Palta, Dr. Shiv Parsad, and Dr. Aman George of NDRI, Karnal. The scientists are of the opinion that the embryonic stem cells have better cloning ability as compared to somatic cells, as such the epigenetic reprogramming of these cells is much more efficient than the somatic cells, which are already differentiated and lineage committed.
This cloned calf weighing 32 kg. was born through caesarian operation carried out by a team of doctors comprising Dr. R.S. Bisla from CCSHAU, Hisar and Dr. K.P.S. Tomar, Dr. Subhash Chand and Dr. Parveen Kumar and Dr. M.K. Srivastava from NDRI, Karnal. The animal is apparently normal and healthy.
Earlier NDRI has produced the world’s first cloned buffalo calf on 6th February, 2009. The hand-guided cloning technique developed at NDRI, is an advanced modification of the “Conventional Cloning Technique”. In this technique, immature oocytes were isolated from ovaries and were matured in vitro. These were then denuded and treated with an enzyme to digest the outer layer of oocytes called ‘zona pellucida’. The oocytes were then treated with chemicals to push their genetic material to one side of the oocyte. This protruded side was then cut off with the help of “hand held fine blade” for removing the original genetic material of the oocyte. The enucleated oocyte was then electrofused with single cell taken from colony of embryonic stem cells. The resulting embryos were cultured and grown in the laboratory for seven days to develop them to the stage of blastocyst.  The blastocysts were transferred to recipient buffaloes.
India is home to more than 56% of world buffalo population. Although we have a huge livestock population, we are facing acute shortage of feed and fodder resources. Another major problem is the scarcity of the superior quality of animals and a majority of our buffaloes is low producer. This is posing huge challenges in substantially increasing country's milk production. Out of around 100 million milch cattle and buffaloes in the country, 47 million are buffaloes, 11 million are crossbred cows and 45 million belong to indigenous cattle breeds.

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Kalpana Chawla old pic



Indian-born astronaut Kalpana Chawla, right, stands next to schoolmate Desi Chawla, in an undated photograph taken in their hometown of Karnal and shown to the media in Karnal Haryana, India on Sunday Feb. 2, 2003. While Kalpana Chawla moved from Karnal more than two decades ago she remains a hero to the people who followed her career at NASA that ended with Saturday's space shuttle catastrophe. (AP Photo/Elizabeth Dalziel)

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Proud Of Karnal Kalpana Chawla


Kalpana Chawla
Kalpana Chawla was born in Karnal, Haryana on 1st July, 1961. Her father Banarsi Lal Chawla was then a leading industrialist of Karnal and owned a Tire factory. Her mother Sanyogita Chawla, a housewife, expected a boy as her last child, when Kalpana was born. In 1976, she did her schooling from Tagore Bal Niketan school, Karnal. She did her pre-university and pre-engineering from Dayal Singh College, Karnal. She then graduated with BSc (Engg) degree in Aeronautics from Punjab Engineering College, Chandigarh. Master of science degree in aerospace engineering in 1984 from University of Texas. Doctorate of philosophy in aerospace engineering from University of Colorado, 1988.
PERSONAL DATA:
Born in Karnal, India. Kalpana Chawla enjoys flying, hiking, back-packing, and reading. She holds Certificated Flight Instructor’s license with airplane and glider ratings, Commercial Pilot’s licenses for single- and multi-engine land and seaplanes, and Gliders, and instrument rating for airplanes. She enjoys flying aerobatics and tail-wheel airplanes.
EDUCATION:
Graduated from Tagore School, Karnal, India, in 1976. Bachelor of science degree in aeronautical engineering from Punjab Engineering College, India, 1982. Master of science degree in aerospace engineering from University of Texas, 1984. Doctorate of philosophy in aerospace engineering from University of Colorado, 1988.
EXPERIENCE:
In 1988, Kalpana Chawla started work at NASA Ames Research Center in the area of powered-lift computational fluid dynamics. Her research concentrated on simulation of complex air flows encountered around aircraft such as the Harrier in “ground-effect.” Following completion of this project she supported research in mapping of flow solvers to parallel computers, and testing of these solvers by carrying out powered lift computations. In 1993 Kalpana Chawla joined Overset Methods Inc., Los Altos, California, as Vice President and Research Scientist to form a team with other researchers specializing in simulation of moving multiple body problems. She was responsible for development and implementation of efficient techniques to perform aerodynamic optimization. Results of various projects that Kalpana Chawla participated in are documented in technical conference papers and journals.
NASA EXPERIENCE:
Selected by NASA in December 1994, Kalpana Chawla reported to the Johnson Space Center in March 1995 as an astronaut candidate in the 15th Group of Astronauts. After completing a year of training and evaluation, she was assigned as crew representative to work technical issues for the Astronaut Office EVA/Robotics and Computer Branches. Her assignments included work on development of Robotic Situational Awareness Displays and testing space shuttle control software in the Shuttle Avionics Integration Laboratory.
In November, 1996, Kalpana Chawla was assigned as mission specialist and prime robotic arm operator on STS-87 (November 19 to December 5, 1997). STS-87 was the fourth U.S Microgravity Payload flight and focused on experiments designed to study how the weightless environment of space affects various physical processes, and on observations of the Sun’s outer atmospheric layers. Two members of the crew performed an EVA (spacewalk) which featured the manual capture of a Spartan satellite, in addition to testing EVA tools and procedures for future Space Station assembly. In completing her first mission, Kalpana Chawla traveled 6.5 million miles in 252 orbits of the Earth and logged 376 hours and 34 minutes in space. In January, 1998, Kalpana Chawla was assigned as crew representative for shuttle and station flight crew equipment. Subsequently, she was assigned as the lead for Astronaut Offices Crew Systems and Habitability section. She is currently assigned to the crew of STS-107 scheduled for launch in 2003.

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